TABLE I
Residues under investigation in D1
Residue=>to  Oligo.	Seq.   Plasmid	In 	Pho+	Fluor.rise   Back 	   2-elec. gate
                                     Chlamy.                     reaction
Y161	F       +	+	pBA155	 +	-     very small  presumed rapid    not detect.
P162	L	+	+ 	pBA155	 +	++	NY	      NY	       NY
F180	L	+	+	pBA155	 +	+++	normal	    normal	    normal
F182	L	+	+	pBA155	 +	+++	normal	    normal	    normal
F182	Y	+	+	pBA155	 +	+++	normal	    normal	    normal
F186	L	+	+	pBA155	 +	+	NY	      NY	       NY
E189	Q	+	+	pBA155	 + 	+++	slow?   rapid+norm.phases   normal
E189	L	+	+	pBA158	 +	+++	normal  rapid+norm.phases    ambig
E189	D	+	+	pBA158	 +	-    F1. normal    normal	      ambig
						    F2. truncated -	               -
N191	A	+	+	pBA158	 +	+++	normal	    slow            normal
N191	L	+	+	pBA155	 +	+++	normal	    slow            normal
N191	D	+	+	pBA155	 +	+++	normal	   slowed           normal
H252	Q	+	+	pBA155	 +	-	normal	    normal	  inhibited
S264	G	+	+	pBA155	 +	++	normal	    normal	  perturbed
R257	K,E,M	+	+	pBA158	 +	+++	NY	      NY	       NY 
R257	Q	+	+	pBA158	 +	-	NY	      NY	       NY
Random mutagenesis	NY	pBA158	 +	NA	NY	      NY	       NY
Selectable marker genes co-transformed with pBA155
Marker gene	Plasmid     In Chlamy.	Pho+	Expression 
ErmF		pBA157	+	+++		Yes (ermr)
TetQ		pBA157	+	+++		Weak tetr?
Gfp		pBA157	+	+++		Not detectable
LuxAB		pBA157	NY	NY		NY
Residues under investigation in D2
Residue =>to	Oligo.	Seq.	Plasmid	In Chlamy.	
Y160	F	+	+	pBD102	NY
Y160	L	+	+	pBD102	NY
P161	A	+	NY	pBD102	NY
F179	L	+	NY	pBD102	NY
F181	L	+	NY	pBD102	NY
F185	L	+	NY	pBD102	NY
F188	Y	+	NY	pBD102	NY
N190	D	+	NY	pBD102	NY
Notes: 
Olig, oligonucleotide synthesized; 
Seq, cassette sequenced; 
Plasmid, plasmid used as transformation vehicle; 
Chlamy, C. reinhardtii transformed, with growth on TAP medium; 
Pho+, photosynthetic growth (wild-type rates +++, 
	lower rates indicated by fewer +, - is pho-); 
Fluor. rise, variable fluorescence rise in the sub-micro second time scale; 
Back Reaction, Charge recombination measured by decay of variable fluorescence 
	in the presence of DCMU; 
2-elec. gate, kinetics of QA- oxidation after 1 or 2 flashes, showing binary pattern 
	(normal) or normal kinetics after 1 flash but no binary pattern (ambig); 
NY, not yet completed.